Title: A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes
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| Authors: Johnson, W; Dai, CS; Liu, J; Wang, X; Luu, DK; Zhang, ZR; Ru, CH; Zhou, C; Tan, M; Pu, HY; Xie, SR; Peng, Y; Luo, J; Sun, Y
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| Author Full Names: Johnson, Wesley; Dai, Changsheng; Liu, Jun; Wang, Xian; Luu, Devin K.; Zhang, Zhuoran; Ru, Changhai; Zhou, Chao; Tan, Min; Pu, Huayan; Xie, Shaorong; Peng, Yan; Luo, Jun; Sun, Yu
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| Source: IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 65 (3):678-686; 10.1109/TBME.2017.2713302 MAR 2018
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| Language: English
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| Abstract: Mammalian oocytes such as mouse oocytes have a highly elastic outer membrane, zona pellucida (ZP) that cannot be penetrated without significantly deforming the oocyte, even with a sharp micropipette. Piezo drill devices leverage lateral and axial vibration of the micropipette to accomplish ZP penetration with greatly reduced oocyte deformation. However, existing piezo drills all rely on a large lateral micropipette vibration amplitude (> 20 mu m) and a small axial vibration amplitude (< 0.1 mu m). The very large lateral vibration amplitude has been deemed to be necessary for ZP penetration although it also induces larger oocyte deformation and more oocyte damage. This paper reports on a new piezo drill device that uses a flexure guidance mechanism and a systematically designed pulse train with an appropriate base frequency. Both simulation and experimental results demonstrate that a small lateral vibration amplitude (e.g., 2 mu m) and an axial vibration amplitude as large as 1.2 mu m were achieved. Besides achieving 100% effectiveness in the penetration of mouse oocytes (n = 45), the new piezo device during ZP penetration induced a small oocyte deformation of 3.4 mu m versus larger than 10 mu m using existing piezo drill devices.
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| ISSN: 0018-9294
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| eISSN: 1558-2531
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| IDS Number: FW9OM
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| Unique ID: WOS:000425664500021
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| PubMed ID: 28600237
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